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Your Position: Home > Cytokines > IL-21 > IL1-H5213

Human IL-21 Protein, Tag Free, low endotoxin (HPLC verified)

  • Synonym
    IL21,Za11,Interleukin-21
  • Source
    Human IL-21, Tag Free (IL1-H5213) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 162 (Accession # Q9HBE4-1).
    Predicted N-terminus: Gln 30
  • Molecular Characterization
    Online(Gln 30 - Ser 162) Q9HBE4-1

    This protein carries no "tag".

    The protein has a calculated MW of 15.5 kDa. The protein migrates as 16-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

    >98% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-21, Tag Free (Cat. No. IL1-H5213) SDS-PAGE gel

Human IL-21, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

SEC-HPLC
Human IL-21, Tag Free (Cat. No. ) HPLC images

The purity of Human IL-21, Tag Free (Cat. No. IL1-H5213) was greater than 98% as determined by SEC-HPLC.

Bioactivity-ELISA
Human IL-21, Tag FreeHuman IL-21, Tag Free (Cat. No. IL1-H5213) ELISA bioactivity

Immobilized Human IL-21, Tag Free (Cat. No. IL1-H5213) at 5 μg/mL (100 μL/well) can bind Human IL-21 R, His Tag (Cat. No. ILR-H5226) with a linear range of 20-78 ng/mL (QC tested).

Human IL-21, Tag FreeHuman IL-21, Tag Free (Cat. No. IL1-H5213) ELISA bioactivity

Immobilized Human IL-21, Tag Free (Cat. No. IL1-H5213) at 5 μg/mL (100 μL/well) can bind Human IL-21 R, Fc Tag (Cat. No. ILR-H5257) with a linear range of 5-39 ng/mL (Routinely tested).

  • Background
    Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
  • Clinical and Translational Updates
      
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