This protein carries no "tag".
The protein has a calculated MW of 15.5 kDa. The protein migrates as 16-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The sterility testing was performed by membrane filtration method described in USP<71>and Eur. Ph. 2.6.1.
>98% as determined by SDS-PAGE.
>98% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-21, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
The purity of Human IL-21, Tag Free (Cat. No. IL1-H5213) was greater than 98% as determined by SEC-HPLC.
Human IL-21, Tag Free (Cat. No. IL1-H5213) immobilized on CM5 Chip can bind Human IL-21 R, Fc Tag (Cat. No. ILR-H5257) with an affinity constant of 0.412 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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