0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Receptor Tyrosine Kinases > Her2 > HE2-HP2E3

PE-Labeled Human Her2 / ErbB2 Protein, His Tag (Site-specific conjugation)

  • Synonym
    ERBB2,CD340,HER-2,neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    PE-Labeled Human Her2, His Tag (HE2-HP2E3) is produced via conjugation of PE to Human Her2, His Tag under optimal conditions. Human Her2, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
    Predicted N-terminus: Thr 23
  • Molecular Characterization
    Online(Thr 23 - Thr 652) P04626-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 73.0 kDa.

  • Application
    Evaluation of anti-HER2 CAR expression by flow cytometry (Please note that this product is NOT compatible to streptavidin detection system).
  • Conjugate

    PE

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

5e5 of anti-Her2 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human Her2, His Tag (Cat. No. HE2-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

  • Background
    Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $319.00

Price(USD) : $1980.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:24 Details
  • Number of Drugs in Clinical Trials:126 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop