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Your Position: Home > Immune Checkpoint Proteins > CD30 > CD0-HP2E3

PE-Labeled Human CD30 / TNFRSF8 Protein, His Tag (Site-specific conjugation)

  • Synonym
    TNFRSF8,CD30,D1S166E,Ki-1
  • Source
    PE-Labeled Human CD30, His Tag (CD0-HP2E3) is produced via site-specific conjugation of PE to Human CD30, His Tag under optimal conditions with a proprietary technology. Human CD30, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    Online(Phe 19 - Lys 379) NP_001234.2

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.1 kDa.

  • Application
    Evaluation of anti-CD30 CAR expression by flow cytometry (Please note that this product is NOT compatible to streptavidin detection system).
  • Conjugate

    PE

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

5e5 of anti-CD30 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD30, His Tag (Cat. No. CD0-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

  • Background
    Human CD30 is also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $319.00

Price(USD) : $1980.00

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  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:14 Details
  • Latest Research Phase:Approved

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