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Your Position: Home > Viral Proteins > Nucleocapsid protein > NUN-C52Hc

SARS-CoV-2 Nucleocapsid protein (P80R), His Tag

  • Synonym
    Nucleocapsid protein,NP,Protein N
  • Source
    SARS-CoV-2 Nucleocapsid protein (P80R), His Tag (NUN-C52Hc) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P80R)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutation P80R was identified on the nucleocapsid protein of SARS-CoV-2 variants with high frequency in P.1 variants circulating in Brazil (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Online(Met 1 - Ala 419) QHO62115.1 (P80R)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 47.4 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 Nucleocapsid protein (P80R), His Tag (Cat. No. NUN-C52Hc) SDS-PAGE gel

SARS-CoV-2 Nucleocapsid protein (P80R), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
SARS-CoV-2 Nucleocapsid protein (P80R), His TagSARS-CoV-2 Nucleocapsid protein (P80R), His Tag (Cat. No. NUN-C52Hc) ELISA bioactivity

Immobilized SARS-CoV-2 Nucleocapsid protein (P80R), His Tag (Cat. No. NUN-C52Hc) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-M223)) with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • References
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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