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Your Position: Home > All Other Proteins > IL-2 R beta & IL-2 R gamma > ILG-R5283

Rat IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified)

  • Synonym
    IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG
  • Source
    Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (ILG-R5283) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Glu 239 (IL-2RB) & Ser 25 - Ala 262 (IL-2RG) (Accession # P26896-1(IL-2RB) & Q68FU6-1(IL-2RG)).
    Predicted N-terminus: Ala 27 (IL-2RB) & Ser 25 (IL-2RG)
  • Molecular Characterization
    Online(Ala 27 - Glu 239 (IL-2RB) & Ser 25 - Ala 262 (IL-2RG)) P26896-1 (IL-2RB) & Q68FU6-1 (IL-2RG)

    Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 30.2 kDa (IL-2RB) and 34.7 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 40-50 kDa and 55-65 kDa respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-R5283) SDS-PAGE gel

Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ) MALS images

The purity of Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-R5283) was more than 85% and the molecular weight of this protein is around 85-100 kDa verified by SEC-MALS.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) on SA Biosensor, can bind Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-R5283) with an affinity constant of 3.50 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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