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Your Position: Home > 2019-nCoV proteins > Spike S1 > RAS-T001

Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike S1)

For research use only.
Materials Provided
IDComponentsSize
RAS001-C01Pre-coated SARS-CoV-2 Spike S1 Microplate1 plate
RAS001-C02Positive Control100 μL
RAS001-C03Negative Control100 μL
RAS001-C04HRP-Anti-Human IgG200 μL
RAS001-C0510xWashing Buffer 50 mL
RAS001-C06Dilution Buffer50 mL
RAS001-C07Substrate Solution12 mL
RAS001-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
    It is for research use only.
  • Storage

    The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 Spike protein S1. The kit consists of High-bind Plate, Spike protein S1, an Positive control, Negative Control, an HRP-Anti-Human IgG secondary antibody and Blocking/Dilution buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike S1)Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike S1) (Cat. No. RAS-T001) ELISA bioactivity

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike S1 at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG in 1:400 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 0.24 ng/mL (QC tested).

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Price(USD) : $580.00

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