This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 14.6 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human R-Spondin 1 (21-146), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Human EGF (Cat. EGF-H52H3), Noggin (Cat. NON-H5257) and R-spondin1 (Cat. RS6-H4220) could support the growth of mouse intestinal organoids.
Immobilized Human R-Spondin 1 (21-146), His Tag (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.01-0.156 μg/mL (QC tested).
Immobilized Human R-Spondin 1 (21-146), His Tag (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 0.039-2.5 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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