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SARS-CoV-2 Spike RBD (B.1.351) Coupled Magnetic Beads

Materials Provided
ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>1000 pmol (28 μg) SARS-CoV-2 RBD protein/mg beads>1000 pmol (28 μg) SARS-CoV-2 RBD protein/mg beads
Binding Capacity>300 pmol (33 μg) Human ACE2 ligand/mg beads>300 pmol (33 μg) Human ACE2 ligand/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)
  • Background
    The SARS-CoV-2 Spike RBD (B.1.351) Coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike RBD to streptavidin-conjugated magnetic beads. The spike RBD coupled to the beads contains the critical mutations (K417N, E484K, N501Y) identified in the South African variant (known as B.1.351 or 20C/501Y.V2). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
  • Reconstitution
    See Certificate of Analysis (CoA) for detailed instruction.
  • Storage

    Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.

    Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

  • Assay Principles
    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

    Antibody Purification:

    1. Resuspend the lyophilized beads by adding the buffer of choice.

    2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

    3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

    4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

Typical Data
SARS-CoV-2 Spike RBD (B.1.351) Coupled Magnetic BeadsSARS-CoV-2 Spike RBD (B.1.351) Coupled Magnetic Beads (Cat. No. MBS-K032) ELISA bioactivity

Immobilized 40 μg RBD protein (Cat. No. SPD-C82E5) /1mg beads can bind ACE2 (Cat. No. AC2-H5257) with an EC50 of 2.395 μg/mL (QC tested).

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Price(USD) : $800.00

Price(USD) : $2500.00

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