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| Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Physical appearance | Powder mixture | Powder mixture |
| Amount of Coupled Protein | ≈294 pmol (41 μg) SARS-CoV-2 Spike Trimer (B.1.351)/mg beads | ≈294 pmol (41 μg) SARS-CoV-2 Spike Trimer (B.1.351)/mg beads |
| Binding Capacity | >126 pmol (19 μg) Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody/mg beads | >126 pmol (19 μg) Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody/mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
See Certificate of Analysis (CoA) for detailed instruction.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
Immobilized 41μg S protein trimer/1mg beads can bind the Anti-S1 Antibody with an EC50 of 0.5951μg/mL.
Accelerated stability test. After placing the lyophilized beads at 37°C for 7 days, binding activity between the SARS-CoV-2 Spike Trimer (B.1.351) Coupled Magnetic Beads (Cat.No. MBS-K030) and anti-SARS-CoV-2 Spike S1 antibody showed little deviation from the unaccelerated sample(%RSD<5%). Data were measured on day 0, 3, 7 respectively.
Freeze-thaw stability test. After different freeze-thaw cycles, binding activity between SARS-CoV-2 Spike Trimer (B.1.351) Coupled Magnetic Beads (Cat.No. MBS-K030) and anti-SARS-CoV-2 Spike S1 antibody showed little deviation from the unfree-thaw sample (%RSD<5%). Three freeze-thaw cycles were performed.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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