The Human HGF R-coupled Magnetic Beads is produced by coupling biotinylated HGF R protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-HGF R antibody or HGF from your sample with high specificity.
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
See Certificate of Analysis (CoA) for detailed instruction.
Upon receipt, please store the beads at -20℃ for 1 year in lyophilized state.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
Immobilized 61.5 μg S2 protein to 1 mg Beads can bind the Anti-SARS-CoV-2 S2 Antibody with an EC50 of 0.4837 μg/mL (QC tested).