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Your Position: Home > Cytokines > IL23A & IL12B > ILB-HR52W3

Human IL-23 alpha & Rat IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)

  • Synonym
    IL-23 alpha & IL-12 beta
  • Source
    Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (ILB-HR52W3) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9NPF7-1(IL23A) & Q9R278-1(IL12B)).
    Predicted N-terminus: Arg 20 (IL23A) & Met 23 (IL12B)
  • Molecular Characterization
    Online(Arg 20 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B)) Q9NPF7-1 (IL23A) & Q9R278-1 (IL12B)

    Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.6 kDa (IL-23A) and 35.9 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 23 kDa (IL-23A) and 45 kDa and 47 kDa (IL-12B) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HR52W3) SDS-PAGE gel

Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ) MALS images

The purity of Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HR52W3) was more than 90% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag FreeHuman IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HR52W3) ELISA bioactivity

Immobilized Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HR52W3) with a linear range of 0.039-0.156 μg/mL (QC tested).

Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag FreeHuman IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HR52W3) ELISA bioactivity

Immobilized Human IL-23A & Rat IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HR52W3) at 5 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 5-78 ng/mL (Routinely tested).

  • Background
    Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $3500.00

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