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Your Position: Home > Membrane Protein > GPRC5D > GPD-H52D4

Human GPRC5D Protein, Flag,His Tag (Nanodisc)

  • Synonym
    GPRC5D
  • Source
    Human GPRC5D, Flag,His Tag (Nanodisc) (GPD-H52D4) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Phe 192 & Glu 197 - Leu 262 (Accession # Q9NZD1-2).
    Predicted N-terminus: Asp
  • Molecular Characterization
    Online(Tyr 2 - Phe 192 & Glu 197 - Leu 262) Q9NZD1-2

    This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus with calculated MW of 51.8 kDa and migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;

    2. -70°C for 3 months under sterile conditions.

  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow this link for product information.
SDS-PAGE
Human GPRC5D, Flag,His Tag (Nanodisc) (Cat. No. GPD-H52D4) SDS-PAGE gel

Human GPRC5D, Flag,His Tag (Nanodisc) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human GPRC5D, Flag,His Tag (Nanodisc)Human GPRC5D, Flag,His Tag (Nanodisc) (Cat. No. GPD-H52D4) ELISA bioactivity

Immobilized Human GPRC5D, Flag,His Tag (Nanodisc) (Cat. No. GPD-H52D4) at 10 μg/mL (100 μL/well) can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.01-0.08 μg/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-GPRC5D Antibody captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human GPRC5D, Flag,His Tag (Nanodisc) (Cat. No. GPD-H52D4) with an affinity constant of 0.179 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Anti-GPRC5D Antibody, Human IgG4 on Protein A Biosensor, can bind Human GPRC5D, Flag,His Tag (Nanodisc) (Cat. No. GPD-H52D4) with an affinity constant of 5.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N terminal extracellular region. Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.
  • Clinical and Translational Updates

      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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