|TAS025-C01||Pre-coated SARS-CoV-2 Spike Protein Microplate||1 plate|
|TAS025-C02||Positive Control||100 μL|
|TAS025-C03||Negative Control||100 μL|
|TAS025-C04||HRP-Anti-Human IgG||200 μL|
|TAS025-C05||10xWashing Buffer||50 mL|
|TAS025-C06||Dilution Buffer||50 mL|
|TAS025-C07||Substrate Solution||12 mL|
|TAS025-C08||Stop Solution||7 mL|
The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add the diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:400 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 48 ng/mL (QC tested).
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