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Your Position: Home > Kits > Spike protein > TAS-K025

Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

For research use only.
Materials Provided
IDComponentsSize
TAS025-C01Pre-coated SARS-CoV-2 Spike Protein Microplate1 plate
TAS025-C02Positive Control100 μL
TAS025-C03Negative Control100 μL
TAS025-C04HRP-Anti-Human IgG200 μL
TAS025-C0510xWashing Buffer50 mL
TAS025-C06Dilution Buffer50 mL
TAS025-C07Substrate Solution12 mL
TAS025-C08Stop Solution7 mL
  • Background
    The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health, and it spreads quickly.This kit is developed for detection of IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
  • Application
    This kit is developed for serologic test of anti-SARS-CoV-2 antibody (Spike Trimer) IgG titer in serum/plasma in vitro.
    It is for research use only.
  • Storage

    The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 Spike Protein. The kit consists of Pre-coated SARS-CoV-2 Spike Microplate, SARS-CoV-2 Spike Protein, an Anti-SARS-CoV-2 Antibody (Control, IgG), an HRP-Anti-Human IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps: a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add the diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer) (Cat. No. TAS-K025) ELISA bioactivity

Detection of Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:400 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 48 ng/mL (QC tested).

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Price(USD) : $580.00

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