|Items||Size （2mg）||Size（5mg X 2）|
|Particle size||2 μm||2 μm|
|Physical appearance||Powder mixture||Powder mixture|
|Amount of Coupled Protein||>1121 pmol (25 μg) VEGF165/mg Beads||>1121 pmol (25μg) VEGF165/mg Beads|
|Binding Capacity||>137 pmol (20μg) Anti-VEGF antibody/mg beads||>137 pmol (20 μg) Anti-VEGF antibody/mg beads|
|Formulation||PBS, pH7.4, with 10% Trehalose||PBS, pH7.4, with 10% Trehalose|
|Reconstitution||2 mL ultrapure water (1 mg beads/mL)||5 mL ultrapure water (1 mg beads/mL)|
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
Immobilized 31.6 μg VEGF165 protein/1mg beads can bind Anti-VEGF Antibody with an EC50 of 0.7726 μg/mL (QC tested).
Accelerated stability test. After placing the lyophilized beads at 37℃ for 7 days, binding activity between the Human VEGF165-coupled Magnetic Beads (Cat.No. MBS-K036) and Bevacizumab showed little deviation from the unaccelerated sample (%RSD<10%). Data were measured on day 0, 3, 7 respectively.
Freeze-thaw stability test. After different freeze-thaw cycles, binding activity between the Human VEGF165-coupled Magnetic Beads (Cat.No. MBS-K036) and Bevacizumab showed little deviation from the unfree-thaw sample (%RSD<10%). Three freeze-thaw cycles were performed.
Price(USD) : $800.00
Price(USD) : $2500.00
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