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Your Position: Home > All Other Proteins > VCAM-1 > VC1-H5253

Human VCAM-1 / CD106 Protein, Fc Tag (MALS verified)

  • Synonym
    VCAM1,CD106,INCAM-100,V-CAM 1,VCAM-1
  • Source
    Human VCAM-1, Fc Tag (VC1-H5253) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Glu 698 (Accession # P19320-1).
    Predicted N-terminus: Phe 25
  • Molecular Characterization
    Online(Phe 25 - Glu 698) P19320-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 100.5 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human VCAM-1, Fc Tag (Cat. No. VC1-H5253) SDS-PAGE gel

Human VCAM-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human VCAM-1, Fc Tag (Cat. No. ) MALS images

The purity of Human VCAM-1, Fc Tag (Cat. No. VC1-H5253) was more than 85% and the molecular weight of this protein is around 235-265 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human VCAM-1, Fc TagHuman VCAM-1, Fc Tag (Cat. No. VC1-H5253) ELISA bioactivity

Immobilized Human VCAM-1, Fc Tag (Cat. No. VC1-H5253) at 5 μg/mL (100 μL/well) can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with a linear range of 5-156 ng/mL (QC tested).

Human VCAM-1, Fc TagHuman VCAM-1, Fc Tag (Cat. No. VC1-H5253) ELISA bioactivity

Immobilized Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human VCAM-1, Fc Tag (Cat. No. VC1-H5253) with a linear range of 0.3-2 ng/mL (Routinely tested).

  • Background
    Vascular cell adhesion protein 1 (VCAM1) is also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. VCAM1 / CD106 contains 7 Ig-like C2-type (immunoglobulin-like) domains. CD106 / VCAM-1 is expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. L1CAM / VCAM-1 is Important in cell-cell recognition and appears to function in leukocyte-endothelial cell adhesion. CD106 / VCAM1 interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1 / VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. INCAM-100 / VCAM1 binds to ECMV-D capsid proteins and acts as a receptor for this virus.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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  • Number of Launched Drugs:1 Details
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  • Latest Research Phase:Approved

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