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Your Position: Home > All Other Proteins > TACI > TAI-H5253

Human TACI / TNFRSF13B (R119A, R122A) Protein, Fc Tag (MALS verified)

  • Synonym
    CD267,TACI,TNFRSF13B
  • Source
    Human TACI (R119A, R122A), Fc Tag (TAI-H5253) is expressed from human 293 cells (HEK293). It contains AA Ser 2 - Thr 166 (Accession # O14836-1(R119A, R122A)).
    Predicted N-terminus: Ser 2
  • Molecular Characterization
    Online(Ser 2 - Thr 166) O14836-1 (R119A,R122A)

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 44.8 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) SDS-PAGE gel

Human TACI (R119A, R122A), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human TACI (R119A, R122A), Fc Tag (Cat. No. ) MALS images

The purity of Human TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) was more than 90% and the molecular weight of this protein is around 45-55 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human TACI (R119A, R122A), Fc TagHuman TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) ELISA bioactivity

Immobilized Human BAFF, His Tag (Cat. No. BAF-H5248) at 2 μg/mL (100 μL/well) can bind Human TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) with a linear range of 0.2-2 ng/mL (QC tested).

  • Background
    Transmembrane activator and CAML interactor (TACI), also known as tumor necrosis factor receptor superfamily member 13B (TNFRSF13B). It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. The present study demonstrated that, in NSCLC, a proliferation-inducing ligand (APRIL), B-cell maturation antigen (BCMA)and transmembrane activator and CAML interactor (TACI) proteins are abnormally expressed by immunohistochemistry, reverse transcription-quantitative polymerase chain reaction and western blotting. In addition, the expression of APRIL, BCMA and TACI were observed to be involved in extracellular signal-regulated kinase (ERK)1/2 activation in A549 cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $290.00

Price(USD) : $1910.00

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