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Your Position: Home > All Other Proteins > IL-2 R beta & IL-2 R gamma > ILG-C82F4

Biotinylated Cynomolgus IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)

  • Synonym
    IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG
  • Source
    Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (ILG-C82F4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Thr 240 (IL-2RB) & Leu 23 - Pro 255 (IL-2RG) (Accession # Q38J85-1(IL-2RB) & Q38JL2-1(IL-2RG)).
    Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)
  • Molecular Characterization

    Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB andIL-2RG, has a calculated MW of 53.0 kDa (IL-2RB) and 55.4 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The reducing (R) protein migrates as 70-85 kDa and 90-110 kDa respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F4) SDS-PAGE gel

Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F4) was more than 90% and the molecular weight of this protein is around 150-170 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,AvitagBiotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F4) ELISA bioactivity

Immobilized Human IL-2, Tag Free (Cat. No. IL2-H4113) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque OX40 Ligand, Mouse IgG2a Fc Tag (Cat. No. ILG-C82F4) with a linear range of 0.02-0.625 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (Cat. No. ILG-C82F4) on SA Biosensor, can bind Human IL-2, Tag Free (Cat. No. IL2-H4113) with an affinity constant of 2.34 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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