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Your Position: Home > Anti-idiotypic Antibodies > Rituximab > RIB-Y35c

Anti-Rituximab Antibodies (MALS verified)

  • Source
    Anti-Rituximab Antibodies (RIB-Y35c) are expressed from human 293 cells (HEK293).
  • Isotype
    IgG1 / kappa
  • Specificity
    Recognizes Rituximab specifically, no cross reactivity with other humanized antibodies.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 years under sterile conditions after reconstitution.
Anti-Rituximab Antibodies (Cat. No. RIB-Y35c) SDS-PAGE gel

Anti-Rituximab Antibodies on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Anti-Rituximab Antibodies (Cat. No. ) MALS images

The purity of Anti-Rituximab Antibodies (Cat. No. RIB-Y35c) was more than 90% and the molecular weight of this protein is around 135-155kDa verified by SEC-MALS.

Anti-Rituximab AntibodiesAnti-Rituximab Antibodies (Cat. No. RIB-Y35c) ELISA bioactivity

Immobilized Rituximab at 1 μg/mL (100 μL/well) can bind Anti-Rituximab Antibodies (Cat. No. RIB-Y35c) with a linear range of 0.2-6 ng/mL (QC tested).

Anti-Rituximab AntibodiesAnti-Rituximab Antibodies (Cat. No. RIB-Y35c) ELISA bioactivity

Immobilized Rituximab at 2 μg/mL, add increasing concentrations of Anti-Rituximab Antibodies (Cat. No. RIB-Y35c) in the 10% human serum and then add Anti-CD20, Human IgG1 at 5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 100 ng/mL (QC tested).

  • Background
    Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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