>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Rituximab Antibodies on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-Rituximab Antibodies (Cat. No. RIB-Y35c) is more than 90% and the molecular weight of this protein is around 135-155kDa verified by SEC-MALS.
Immobilized Rituximab at 1 μg/mL (100 μL/well) can bind Anti-Rituximab Antibodies (Cat. No. RIB-Y35c) with a linear range of 0.2-6 ng/mL (QC tested).
Immobilized Rituximab at 2 μg/mL, add increasing concentrations of Anti-Rituximab Antibodies (Cat. No. RIB-Y35c) in the 10% human serum and then add Anti-CD20, Human IgG1 at 5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 100 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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