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Your Position: Home > Cytokines > LIF > LIF-R52H3

Rat LIF Protein, His Tag (MALS verified)

  • Synonym
    LIF,CDF,DIA,HILDA,MLPLI
  • Source
    Rat LIF, His Tag (LIF-R52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # P17777-1).
    Predicted N-terminus: Ser 23
  • Molecular Characterization
    Online(Ser 23 - Phe 202) P17777-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat LIF, His Tag (Cat. No. LIF-R52H3) SDS-PAGE gel

Rat LIF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Rat LIF, His Tag (Cat. No. ) MALS images

The purity of Rat LIF, His Tag (Cat. No. LIF-R52H3) was more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

  • Background
    Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $2380.00

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  • Latest Research Phase:Preclinical

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