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Your Position: Home > Protein-coupled magnetic beads > TROP-2 > MBS-K028

Human TROP-2-coupled Magnetic Beads

Materials Provided
ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>791 pmol (25μg) TROP-2/mg beads>791 pmol (25μg) TROP-2/mg beads
Binding Capacity>1266 pmol (40μg) Anti-trop-2 antibody/mg beads>1266 pmol (40μg) Anti-trop-2 antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)
  • Background
    The Human TROP-2-coupled Magnetic Beads is produced by coupling biotinylated TROP-2 heterodimer to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-TROP-2 antibody from your sample with high specificity.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry.
  • Reconstitution
    See Certificate of Analysis (CoA) for detailed instruction.
  • Storage

    Upon receipt, please store the beads at -20℃ for 1 year in lyophilized state.

    Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

  • Assay Principles
    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

    Antibody Purification:

    1. Resuspend the lyophilized beads by adding the buffer of choice.

    2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

    3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

    4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

Typical Data
Human TROP-2-coupled Magnetic BeadsHuman TROP-2-coupled Magnetic Beads (Cat. No. MBS-K028) ELISA bioactivity

Immobilized 32.6 μg TROP-2/1 mg beads can bind Anti-TROP-2 mAb with an EC50 of 0.8505 μg/mL (QC tested).

Human TROP-2-coupled Magnetic BeadsHuman TROP-2-coupled Magnetic Beads (Cat. No. MBS-K028) ELISA bioactivity

Accelerated stability test. After placing the lyophilized beads at 37℃ for 7 days, binding activity between the Human TROP-2-coupled Magnetic Beads (Cat.No. MBS-K028) and anti-trop-2 mAb showed little deviation from the unaccelerated sample (%RSD<10%). Data were measured on day 0, 3, 7 respectively.

Human TROP-2-coupled Magnetic BeadsHuman TROP-2-coupled Magnetic Beads (Cat. No. MBS-K028) ELISA bioactivity

Freeze-thaw stability test. After different freeze-thaw cycles, binding activity between the Human TROP-2-coupled Magnetic Beads (Cat.No. MBS-K028) and anti-trop-2 mAb showed little deviation from the unfreeze-thaw sample (%RSD<10%). Three freeze-thaw cycles were performed.

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Price(USD) : $800.00

Price(USD) : $2500.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Approved

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