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Your Position: Home > CAR-T > EGFRvIII > EGI-H5255

Human EGFRvIII Protein, Fc Tag (MALS verified)

  • Synonym
    EGFRvIII
  • Source
    Human EGFRvIII, Fc Tag (EGI-H5255) is expressed from human 293 cells (HEK293). It contains AA Leu 25- Ser 378 (Accession # NP_001333870.1).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25- Ser 378) NP_001333870.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 65.1 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human EGFRvIII, Fc Tag (Cat. No. EGI-H5255) SDS-PAGE gel

Human EGFRvIII, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human EGFRvIII, Fc Tag (Cat. No. ) MALS images

The purity of Human EGFRvIII, Fc Tag (Cat. No. EGI-H5255) is more than 90% and the molecular weight of this protein is around 150-184 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human EGFRvIII, Fc TagHuman EGFRvIII, Fc Tag (Cat. No. EGI-H5255) ELISA bioactivity

Immobilized Human EGFRvIII, Fc Tag (Cat. No. EGI-H5255) at 2 μg/mL (100 μL/well) can bind Anti-EGFRvIII Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (QC tested).

  • Background
    The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The type III EGF deletion-mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors; EGFR gene amplification is correlated with the structural rearrangement of the gene. The EGFRvIII gene has an in-frame deletion of 801 base pairs, corresponding to exons 2–7 in the mRNA, resulting in the deletion of amino acids 30-297 in the extracellular domain and the generation of a glycine at the fusion point
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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