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Your Position: Home > Immune Checkpoint Proteins > BTN2A1 > BT1-H52F5

Biotinylated Human BTN2A1 Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    BK14H9.1,BT2.1,BTF1,BTF1BT2.1butyrophilin BTF1,BTN2A1,butyrophilin subfamily 2 member A1,butyrophilin,subfamily 2,member A1,DJ3E1.1,FLJ36567
  • Source
    Biotinylated Human BTN2A1, Fc,Avitag (BT1-H52F5) is expressed from human 293 cells (HEK293). It contains AA Gln 29 - Ala 248 (Accession # Q7KYR7-2).
    Predicted N-terminus: Gln 29
  • Molecular Characterization
    Online(Gln 29 - Ala 248) Q7KYR7-2

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 52.7 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human BTN2A1, Fc,Avitag (Cat. No. BT1-H52F5) SDS-PAGE gel

Biotinylated Human BTN2A1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human BTN2A1, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human BTN2A1, Fc,Avitag (Cat. No. BT1-H52F5) was more than 90% and the molecular weight of this protein is around 114-139 kDa verified by SEC-MALS.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Datasheet & Documentation

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DMF (Drug Master File)

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