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SPN-C52HB-50ug
$465.00
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SPN-C52HB-500ug
$3,170.00
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SPN-C52HB
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  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources

  • 分子别名（Synonym）

    Spike,S protein,Spike glycoprotein,S glycoprotein

  • 表达区间及表达系统（Source）

    SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Hb) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations C136F, Y144del, R190S, D215G, LA242-243del, Y449H, E484K, N501Y, D614G, H655Y, N679K, T716I, T859N were identified in the SARS-CoV-2 variant C.1.2. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

    Predicted N-terminus: Val 16

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 137.6 kDa. The protein migrates as 150-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度（Purity）

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.