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Your Position: Home > Immune Checkpoint Proteins > CD40 Ligand > CDL-M82H5

Biotinylated Mouse CD40 Ligand / TNFSF5 Protein, His,Avitag™ (active trimer) (MALS verified)

  • Synonym
    CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
  • Source
    Biotinylated Mouse CD40 Ligand, His,Avitag (CDL-M82H5) is expressed from human 293 cells (HEK293). It contains AA Gly 115 - Leu 260 (Accession # P27548-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Gly 115 - Leu 260) P27548-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 19.6 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. CDL-M82H5) SDS-PAGE gel

Biotinylated Mouse CD40 Ligand, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. CDL-M82H5) was more than 90% and the molecular weight of this protein is around 56-68 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Mouse CD40 Ligand, His,AvitagBiotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. CDL-M82H5) ELISA bioactivity

Immobilized Mouse CD40, His Tag (Cat. No. TN5-M52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse CD40 Ligand, His,Avitag (Cat. No. CDL-M82H5) with a linear range of 0.5-16 ng/mL (QC tested).

  • Background
    CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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  • Number of Drugs in Clinical Trials:11 Details
  • Latest Research Phase:Phase 3 Clinical

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