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Your Position: Home > Agonist > OX40 Ligand > OXL-H5266

Human OX40 Ligand / TNFSF4 Protein, Fc Tag (active trimer) (MALS verified)

  • Synonym
    OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
  • Source
    Human OX40 Ligand, Fc Tag (OXL-H5266) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # P23510-1).
    Predicted N-terminus: Pro
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 74.0 kDa. The protein migrates as 130-210 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) SDS-PAGE gel

Human OX40 Ligand, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human OX40 Ligand, Fc Tag (Cat. No. ) MALS images

The purity of Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) is more than 90% and the molecular weight of this protein is around 160-196 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human OX40 Ligand, Fc TagHuman OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) ELISA bioactivity

Immobilized Human OX40 Protein, His Tag (MALS verified) (Cat. No. OX0-H5224) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) with a linear range of 0.1-1 ng/mL (QC tested).

Human OX40 Ligand, Fc TagHuman OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) ELISA bioactivity

Immobilized Cynomolgus / Rhesus macaque OX40, His Tag (Cat. No. OX0-C5220) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) with a linear range of 0.2-3 ng/mL (Routinely tested).

Human OX40 Ligand, Fc TagHuman OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) ELISA bioactivity

Immobilized Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human OX40 Ligand, Fc Tag (Cat. No. OXL-H5266) with a linear range of 0.1-0.4 ng/mL (Routinely tested).

  • Background
    Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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