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Your Position: Home > CKP > BTN3A1 > BT1-C52H3

Cynomolgus BTN3A1 / CD277 Protein, His Tag

  • Synonym
    BTN3A1,CD277,BTF5
  • Source
    Cynomolgus BTN3A1, His Tag (BT1-C52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 1 - Ser 218 (Accession # A0A330KVC6-1).
    Predicted N-terminus: Gln 1
  • Molecular Characterization
    Online(Gln 1 - Ser 218) A0A330KVC6-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.4 kDa. The protein migrates as 30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus BTN3A1, His Tag (Cat. No. BT1-C52H3) SDS-PAGE gel

Cynomolgus BTN3A1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Butyrophilin subfamily 3 member A1 (BTN3A1) is also known as CD277 and BTF5, which belongs to the immunoglobulin superfamily and contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. Also, BTN3A1 regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Furthermore, BTN3A1 mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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