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Your Position: Home > 2019-nCoV proteins > B7-H3 > MBS-K040

Human B7-H3(4Ig)-coupled Magnetic Beads

Materials Provided
ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>398 pmol (20μg) Human B7-H3(4Ig)/mg beads>398 pmol (20μg) Human B7-H3(4Ig)/mg beads
Binding Capacity>596 pmol (30μg) Anti-B7-H3 Ab/mg beads>596 pmol (30μg) Anti-B7-H3 Ab/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)
  • Background
    The Human B7-H3-coupled Magnetic Beads is produced by coupling biotinylated human B7-H3 protein to streptavidin-conjugated magnetic beads. The pre-coupled beads are ready to use for capturing anti-B7-H3 antibody from your sample with high specificity.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
  • Reconstitution
    See Certificate of Analysis (CoA) for detailed instruction.
  • Storage

    Upon receipt, please store the beads at -20℃ for 1 year in lyophilized state.

    Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

  • Assay Principles
    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

    Antibody Purification:

    1. Reconstitute the beads with ultrapure water to 1 mg/mL, and wash the beads for three times.

    2. Dilute the biotinalyted protein or antibodies with Assay Buffer and add to the beads. Incubate for 60 minutes at room temperature in a rotary mixer.

    3. Wash the beads for four times, and add Assay Buffer to the beads. The beads are ready to use for the next experiment.

Typical Data
Human B7-H3-coupled Magnetic BeadsHuman B7-H3-coupled Magnetic Beads (Cat. No. MBS-K040) ELISA bioactivity

Immobilized 22.7 μg B7-H3(4Ig) to 1mg Beads can bind the Anti B7H3 Ab with(Cat. No. B73-M51) an EC50 of 1.039 μg/mL (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $800.00

Price(USD) : $2500.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:14 Details
  • Latest Research Phase:Phase 3 Clinical

Datasheet & Documentation

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DMF (Drug Master File)

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