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Your Position: Home > Beads > Nectin-4 > MBS-K039

Human Nectin-4-coupled Magnetic Beads

Materials Provided
ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>529 pmol (20μg) Human Nectin-4/mg beads>529 pmol (20μg) Human Nectin-4/mg beads
Binding Capacity>280 pmol (20μg) Anti-Nectin-4 Ab/mg beads>280 pmol (20μg) Anti-Nectin-4 Ab/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)
  • Background
    The Human Nectin-4-coupled Magnetic Beads is produced by coupling biotinylated human Nectin-4 protein to streptavidin-conjugated magnetic beads. The pre-coupled beads are ready to use for capturing anti-Nectin-4 antibody from your sample with high specificity.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
  • Reconstitution
    See Certificate of Analysis (CoA) for detailed instruction.
  • Storage

    Upon receipt, please store the Beads at -20℃. The shelf life is 6 months at -20 ℃.

    Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

  • Assay Principles
    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

    Antibody Purification:

    1. Reconstitute the beads with ultrapure water to 1 mg/mL, and wash the beads for three times.

    2. Dilute the biotinalyted protein or antibodies with Assay Buffer and add to the beads. Incubate for 60 minutes at room temperature in a rotary mixer.

    3. Wash the beads for four times, and add Assay Buffer to the beads. The beads are ready to use for the next experiment.

Typical Data
Human Nectin-4-coupled Magnetic BeadsHuman Nectin-4-coupled Magnetic Beads (Cat. No. MBS-K039) ELISA bioactivity

Immobilized 24.2 μg Nectin-4 / 1 mg beads can bind Anti-Nectin-4-Ab with an EC50 of 0.5457 μg/mL (QC tested).

Human Nectin-4-coupled Magnetic BeadsHuman Nectin-4-coupled Magnetic Beads (Cat. No. MBS-K039) ELISA bioactivity

Immobilized 24.2 μg Nectin-4 / 1 mg beads can bind Nectin-1 with (Cat. No. PV1-H5253) an EC50 of 0.4625 μg/mL (QC tested).

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

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DMF (Drug Master File)

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