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Your Position: Home > IgM kits > Spike RBD > RAS-T042

Anti-SARS-CoV-2 (B.1.617.2) Antibody IgG Titer Serologic Assay Kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS042-C01Pre-coated SARS-CoV-2 Spike RBD(B.1.617.2) Microplate1 plate
RAS042-C02Positive Control100 μL
RAS042-C03Negative Control100 μL
RAS042-C04HRP-Anti-Human IgG200 μL
RAS042-C0510xWashing Buffer50 mL
RAS042-C06Dilution Buffer50 mL
RAS042-C07Substrate Solution12 mL
RAS042-C08Stop Solution7 mL
  • Background
    Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. The variant B.1.1.7 identified in the United Kingdom (UK) carries a large number of mutations and is reported to spread more easily and quickly than other variants. The variant B.1.351 identified in South Africa ,P.1 identified in Brazil and B.1.617.2 in India show evidence of increased transmissibility and resistance to established immunity. A common mutation identified in the U.K, South African and Brazilian variants is N501Y, and the mutation identified in B.1.617.2 is T478K,which resides on the receptor-binding domain (RBD) of SARS-CoV-2 spike protein. To evaluate the impacts of the new variants, a rapid and effective assay kit detecting the levels of antibody against the mutant is in urgent need.
  • Application
    This Kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.617.2) antibody IgG (Spike RBD) in human serum.
    It is for research use only.
  • Storage

    The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 (B.1.617.2)  Antibody IgG Titer Serologic Assay Kit (Spike RBD)Anti-SARS-CoV-2 (B.1.617.2)  Antibody IgG Titer Serologic Assay Kit (Spike RBD) (Cat. No. RAS-T042) ELISA bioactivity

For determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

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Price(USD) : $650.00

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