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Your Position: Home > All Other Proteins > VCAM-1 > Human VCAM-1 / CD106 Protein, His Tag

Human VCAM-1 / CD106 Protein, His Tag

  • Synonym
    VCAM1,CD106,INCAM-100,V-CAM 1,VCAM-1
  • Source
    Human VCAM-1, His Tag (VC1-H5224) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Pro 697 (Accession # NP_001069).
    Predicted N-terminus: Phe 25
  • Molecular Characterization
    Online(Phe 25 - Pro 697) NP_001069

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 75.0 kDa. The protein migrates as 80-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human VCAM-1, His Tag (Cat. No. VC1-H5224) SDS-PAGE gel

Human VCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA
Human VCAM-1, His TagHuman VCAM-1, His Tag (Cat. No. VC1-H5224) ELISA bioactivity

Immobilized Human VCAM-1, His Tag (Cat. No. VC1-H5224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 0.01-0.156 μg/mL (QC tested).

  • Background
    Vascular cell adhesion protein 1 (VCAM1) is also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. VCAM1 / CD106 contains 7 Ig-like C2-type (immunoglobulin-like) domains. CD106 / VCAM-1 is expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. L1CAM / VCAM-1 is Important in cell-cell recognition and appears to function in leukocyte-endothelial cell adhesion. CD106 / VCAM1 interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1 / VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. INCAM-100 / VCAM1 binds to ECMV-D capsid proteins and acts as a receptor for this virus.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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