Human ADAM9 Protein, His Tag (active enzyme, HPLC verified)

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Cat. No. / Size
Price
Qty
AD9-H52H7-50ug
$550.00
AD9-H52H7-250ug
$1090.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Disintegrin and metalloproteinase domain-containing protein 9 (EC:3.4.24.-) Cellular disintegrin-related protein, Meltrin-gamma, Metalloprotease, disintegrin, cysteine-rich protein 9, Myeloma cell metalloproteinase, ADAM9, KIAA0021, MCMP, MDC9, MLTNG

  • Source

    Human ADAM9, His Tag (AD9-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 206 - Asp 697 (Accession # Q13443-1).

    Predicted N-terminus: Ala 206

    Request for sequence
  • Molecular Characterization

    ADAM9 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.2 kDa. The protein migrates as 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ADAM9 SDS-PAGE

    Human ADAM9, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    ADAM9 SEC-HPLC

    The purity of Human ADAM9, His Tag (Cat. No. AD9-H52H7) was greater than 85% as determined by SEC-HPLC.

  • Bioactivity-ELISA

     ADAM9 ELISA

    Immobilized Human ADAM9, His Tag (Cat. No. AD9-H52H7) at 1 μg/mL (100 μL/well) can bind Izeltabart with a linear range of 0.2-16 ng/mL (QC tested).

    Protocol
  • Bioactivity

    Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >25 pmol/min/µg (QC tested).

    Protocol

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Background

ADAM9 (A disintegrin and a metalloprotease 9) is a membrane-anchored protein that participates in a variety of physiological functions, primarily through the disintegrin domain for adhesion and the metalloprotease domain for ectodomain shedding of a wide variety of cell surface proteins. ADAM9 influences the developmental process, inflammation, and degenerative diseases.Recently, increasing evidence has shown that ADAM9 plays an important role in tumor biology. Overexpression of ADAM9 has been found in several cancer types and is correlated with tumoraggressiveness and poor prognosis. In addition, through either proteolytic or non-proteolytic pathways, ADAM9 promotes tumor progression, therapeutic resistance, and metastasis of cancers.Therefore, comprehensively understanding the mechanism of ADAM9 is crucial for the development of therapeutic anti-cancer strategies. In this review, we summarize the current understanding of ADAM9 in biological function, pathophysiological diseases, and various cancers. Recent advances in therapeutic strategies using ADAM9-related pathways are presented as well.

Recent Advances

 
Drug Development Progress
  • English Name:

    Disintegrin and metalloproteinase domain-containing protein 9

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    3 Details

  • Highest Development Stage:

    Phase 2 Clinical

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