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Your Position: Home > Cytokines > Flt-3 Ligand > FLL-H82E6

Biotinylated Human Flt-3 Ligand Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    FLT3LG,FL,FLT3L,Flt3 ligand
  • Source
    Biotinylated Human Flt-3 Ligand, His,Avitag (FLL-H82E6) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1).
    Predicted N-terminus: Thr 27
  • Molecular Characterization
    Online(Thr 27 - Pro 185) P49771-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 21.6 kDa. The protein migrates as 26-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Flt-3 Ligand, His,Avitag (Cat. No. FLL-H82E6) SDS-PAGE gel

Biotinylated Human Flt-3 Ligand, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human Flt-3 Ligand, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human Flt-3 Ligand, His,Avitag (Cat. No. FLL-H82E6) is more than 90% and the molecular weight of this protein is around 50-68 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human Flt-3 Ligand, His,AvitagBiotinylated Human Flt-3 Ligand, His,Avitag (Cat. No. FLL-H82E6) ELISA bioactivity

Immobilized Human Flt-3, Fc Tag (Cat. No. FL3-H5258) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Flt-3 Ligand, His,Avitag (Cat. No. FLL-H82E6) with a linear range of 0.1-4 ng/mL (QC tested).

  • Background
    FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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