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Your Position: Home > Cytokines > IL-17C > ILC-M82E9

Biotinylated Mouse IL-17C Protein, His,Avitag™ (MALS verified)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    IL-17C,Cytokine CX2,IL17C
  • Source
    Biotinylated Mouse IL-17C, His,Avitag (ILC-M82E9) is expressed from human 293 cells (HEK293). It contains AA Asp 17 - Gln 194 (Accession # Q8K4C5-1).
    Predicted N-terminus: Asp 17
  • Molecular Characterization
    Online(Asp 17 - Gln 194) Q8K4C5-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 23.4 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse IL-17C, His,Avitag (Cat. No. ILC-M82E9) SDS-PAGE gel

Biotinylated Mouse IL-17C, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Mouse IL-17C, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Mouse IL-17C, His,Avitag (Cat. No. ILC-M82E9) is more than 85% and the molecular weight of this protein is around 57-67 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Mouse IL-17C, His,AvitagBiotinylated Mouse IL-17C, His,Avitag (Cat. No. ILC-M82E9) ELISA bioactivity

Immobilized Human IL-17 RE (155-454), Fc Tag (Cat. No. ILE-H5256) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-17C, His,Avitag (Cat. No. ILC-M82E9) with a linear range of 0.2-20 ng/mL (QC tested).

  • Background
    Interleukin-17C(IL-17C) is a glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. IL‑17C binds to IL‑17 RE with high affinity and to IL‑17 RA with low affinity, binds to a heterodimer formed by IL17RA and IL17RE. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G. Synergy is also observed with TNF and IL1B in inducing DEFB2 from keratinocytes.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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