This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 147.6 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE). This protein has two NLS, which leads to transport to the nucleus efficiently. It is ideal for in vitro and in vivo reactions.
The sterility testing was performed by membrane filtration method described in USP<71>and Eur. Ph. 2.6.1.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Supplied as 0.2 μm filtered solution in 20 mM NaAC, 500 mM NaCl, 0.1 mM EDTA, 0.1 mM TCEP, pH6.0 with Glycerol as protectant.
Contact us for customized product form or formulation.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The product MUST be stored at -20°C or lower upon receipt;
-20°C for 3 months under sterile conditions.
NLS-Cas12a Nuclease on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of NLS-Cas12a Nuclease (Cat. No. CAA-L5149) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Measured by its ability to cleave a targeted DNA substrate. Cas12a achieves >90% substrate cleavage (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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