This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 162.4 KDa. The protein migrates as 153±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
≥95% as determined by SEC-MALS.
Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH7.5 with Glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The product MUST be stored at -20°C or lower upon receipt;
-20°C for 3 months under sterile conditions.
GENPower™ NLS-Cas9 Nuclease on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.(With Star Ribbon Pre-stained Protein Marker).
The purity of GENPower™ NLS-Cas9 Nuclease (Cat. No. CA9-S5149) is more than 95% and the molecular weight of this protein is around 140-180kDa verified by SEC-MALS.
GENPower™ NLS-Cas9 Nuclease is evaluated in an in vitro DNA cleavage assay on a DNA fragment containing the target sequence. The activity of the GENPower™ NLS-Cas9 Nuclease is greater than 90% (QC tested).
The cleavage activity of GENPower™ NLS-Cas9 Nuclease in vitro.
The cleavage activity of GENPower™ NLS-Cas9 Nuclease in cell line.
The cleavage activity of GENPower™ NLS-Cas9 Nuclease in human primary T cells.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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