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Your Position: Home > Virus > NS1 > NS1-D52H3

DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (MALS verified)

  • Synonym
    NS1
  • Source
    DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag(NS1-D52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 776 - Ala 1127 (Accession # P29990-1 ).
    Predicted N-terminus: Asp 776
  • Molecular Characterization
    NS1 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 41.8 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with Trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
NS1 SDS-PAGE

DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
NS1 MALS images

The purity of DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag(Cat. No. NS1-D52H3) is more than 90% and the molecular weight of this protein is around 200-225 kDa verified by SEC-MALS.

Bioactivity-ELISA
 NS1 ELISA

Immobilized DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (Cat. No. NS1-D52H3) at 1 μg/mL (100 μL/well) can bind Mouse Anti-NS1(DENV) Antibody 2B7,Mouse IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    Zika virus (ZIKV) is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus. Its name comes from the Zika Forest of Uganda, where the virus was first isolated in 1947. Zika virus is related to dengue, yellow fever, Japanese encephalitis, and West Nile viruses. The infection, known as Zika fever, often causes no or only mild symptoms, similar to a mild form of dengue fever. It is treated by rest. Since the 1950s, it has been known to occur within a narrow equatorial belt from Africa to Asia. As of 2016, the illness cannot be prevented by drugs or vaccines. As of February 2016, there is evidence that Zika fever in pregnant women is associated with abnormal brain development in their fetuses through mother-to-child transmission of the virus, which may result in miscarriage or microcephaly.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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