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Your Position: Home > CD&BIOMARKER > Siglec-7 > SI7-H5258

Human Siglec-7 / CD328 Protein, Fc (L117A, L118A) Tag (MALS verified)

  • Synonym
    CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1
  • Source
    Human Siglec-7, Fc (L117A, L118A) Tag (SI7-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    Online(Gln 19 - Leu 353) Q9Y286-1

    This protein carries a Fc (L117A, L118A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L117A, L118A/EU number: L234A/L235A) in human immunoglobulin G1 (hIgG1) Fc diminish binding to FcγRI leading to a significant reduction in ADCC and CDC in comparison to wild type human IgG1.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-7, Fc (L117A, L118A) Tag (Cat. No. SI7-H5258) SDS-PAGE gel

Human Siglec-7, Fc (L117A, L118A) Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Siglec-7, Fc (L117A, L118A) Tag (Cat. No. ) MALS images

The purity of Human Siglec-7, Fc (L117A, L118A) Tag (Cat. No. SI7-H5258) is more than 85% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Siglec-7, Fc (L117A, L118A) TagHuman Siglec-7, Fc (L117A, L118A) Tag (Cat. No. SI7-H5258) ELISA bioactivity

Immobilized Human Siglec-7, Fc (L117A, L118A) Tag (Cat. No. SI7-H5258) at 5 μg/mL (100 μL/well) can bind Neu5Aca2-6GalNAca-PAA-biotin with a linear range of 0.01-0.625 μg/mL (QC tested).

  • Background
    Siglec-7 is a member of the human CD33‐related Siglec receptor. The extracellular region of Siglec‐7 is characterized by an N‐terminal V‐set Ig domain that can bind sialic acid and two C2‐set Ig domains. The cytoplasmic tail of Siglec‐7 has one immune‐receptor tyrosine‐based inhibitory motif (ITIM) and one ITIM‐like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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