Bat coronavirus Spike Trimer Protein (K978P, V979P), His Tag&Twin-Strep Tag (MALS verified)

分子别名（Synonym）

Spike,S protein,Spike glycoprotein,S glycoprotein

表达区间及表达系统（Source）

Bat coronavirus Spike Trimer, His Tag&Twin-Strep Tag (SPN-B5283) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Gln 1200 (Accession # UAY13253.1 (K978P, V979P)).

Predicted N-terminus: Met 1

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by a twin strep tag.

The protein has a calculated MW of 141.2 kDa. The protein migrates as 170-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程