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Your Position: Home > Enzyme > Nuclease > NUE-S5119

GENIUS™ Nuclease, premium grade

  • Features
    GENIUS™ Nuclease, premium grade is designed for preclinical stage, has more comprehensive QC testing (such as HCP and so on) and tighter specifications based on the following GENIUS features.
    GENIUS™ Nuclease, premium grade has the same activity and performance with GMP Grade Nuclease, which enables a seamless transition from preclinical development to clinical phases. By using GENIUS™ Nuclease, premium grade in your development phase you can easily transit into clinical and commercial phases and avoid needing to amend your manufacturing process and undertake comparability studies.
    1. Generally usable: Generally usable for removing all forms of DNA and RNA from biological products, reducing viscosity and preventing cell clumping.

    2. Efficient: Efficient reaction, rapid degradation, reduce processing time.

    3. Native: Native structure, tag free, the enzyme activity is more guaranteed.

    4. Ideal: Ideal for DNA and RNA clearance

    5. Ultra pure: Ultra pure: High purity and activity

    6. Specific: Specific nuclease, no protease activity

  • Source
    GENIUS™ Nuclease, premium grade is a tag free recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™ Nuclease, premium grade is a homodimer with monomer molecular masses about 30 kDa with an isoelectric point (pI) at pH 6.85. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5’- phosphorylated oligonucleotides of 3-8 bases in length.
  • Operating conditions
    GENIUS™ Nuclease, premium grade is functional between pH 6 and 10 (optimal at pH8 - 8.5) , and from 0°C to 42 °C (optimal at 35 °C - 42 °C). Mg2+ (1-2 mM) is required for enzyme activity.1 mM EDTA reduced the activity by 30% in the presence of 1 mM MgCl2; 0.1 M EDTA eliminated all enzyme activity. In the presence of 1 mM MgCl2, enzyme levels were reduced 75% by 0.1 M CaCl2 or 1 M NaCl. Under standard assay conditions, 1 mM iodoacetate had no effect on the enzymatic rate, whereas 1 mM mercaptoethanol and maleic acid reduced the activity by only 5 to 10%. 10 mM p- Chloromercurybenzoate completely inactivates the enzyme, while 0.64 M beta-mercaptoethanol in the presence of 2 M urea causes only partial inactivation of the enzyme. 4 or 7 M Urea increases the enzyme activity.
  • Application

    Elimination of nucleic acids from recombinant proteins

    1. Preclinical viral vaccine production, which will transit from preclinical development to clinical phases.

    2. Preclinical viral vector production for cell and gene therapy(CGT),which will transit from preclinical development to clinical phases.

    3. Other preclinical development and production uses

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Host Cell Protein

    <0.05ng/ug of protein tested by ELISA.

  • Protease activity

    negative

  • Sterility

    The sterility testing was performed by membrane filtration method.

  • Mycoplasma

    Negative

  • Purity

    >95% as determined by SDS-PAGE.

    >99% as determined by SEC-HPLC.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20mM Tris, 20mM NaCl, 2mM MgCl2, pH 8.0 with 50% Glycerol as protectant.

  • Storage

    Avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;

    2. -20°C for 24 months under sterile conditions.

  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
SDS-PAGE
CD3 epsilon SDS-PAGE

GENIUS™ Nuclease, premium grade on SDS-PAGE under condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
CD3 epsilon MALS images

The purity of GENIUS™ Nuclease, premium grade (Cat.No.NUE-S5119) was greater than 99% as determined by SEC-HPLC.

Bioactivity



Specific activity for GENIUS™ Nuclease, premium grade is measured under standard assay conditions. The specific activity of GENIUS™ Nuclease, premium grade is >1.2 x 10e6 units/mg protein. One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C, which corresponds approximately to complete digestion of 37 μg DNA. Note that 1 KU=1000 units.

GENIUS™ Nuclease, premium grade shows high specific activity.

Stability

The specific activity shows that GENIUS™ Nuclease, premium grade is stable at 4°C for 14 days.

The specific activity shows that GENIUS™ Nuclease, premium grade is stable at 37°C for 48 hours.

Batch consistency

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ETD of in-stock products: 2 business days

Price(USD) : $330.00

Price(USD) : $1260.00

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Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

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