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Your Position: Home > Protein > CLEC-2 > CL2-H5254

Human CLEC-2 / CLEC1B Protein, Fc Tag (MALS & SPR verified)

  • Synonym
    CLEC-2/CLEC1B/C-type Lectin-like Receptor 2
  • Source
    Human CLEC-2, Fc Tag (CL2-H5254) is expressed from human 293 cells (HEK293). It contains AA Ser 55 - Pro 229 (Accession # Q9P126-1).
    Predicted N-terminus: Ser 55
  • Molecular Characterization
    Online(Ser 55 - Pro 229) Q9P126-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 47.2 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CLEC-2, Fc Tag (Cat. No. CL2-H5254) SDS-PAGE gel

Human CLEC-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human CLEC-2, Fc Tag (Cat. No. ) MALS images

The purity of Human CLEC-2, Fc Tag (Cat. No. CL2-H5254) is more than 90% and the molecular weight of this protein is around 100-125 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CLEC-2, Fc TagHuman CLEC-2, Fc Tag (Cat. No. CL2-H5254) ELISA bioactivity

Immobilized Human Podoplanin, His Tag (Cat. No. PON-H52H3) at 5 μg/mL (100 μL/well) can bind Human CLEC-2, Fc Tag (Cat. No. CL2-H5254) with a linear range of 0.313-5 μg/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human Podoplanin, His Tag (Cat. No. PON-H52H3) immobilized on CM5 Chip can bind Human CLEC-2, Fc Tag (Cat. No. CL2-H5254) with an affinity constant of 0.495 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    C-type lectin-like receptor 2 (CLEC-2) is a hemITAM containing receptor that is highly expressed on platelets and regulates many of the non-classical functions of these cells, including in inflammation and infection.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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