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Your Position: Home > Virus > A35R > A3R-M52H3

Monkeypox virus (strain Zaire-96-I-16) A35R Protein, His Tag

  • Synonym
    A35R
  • Source
    Monkeypox virus (strain Zaire-96-I-16) A35R , His Tag (A3R-M52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 58 - Thr 181 (Accession # Q8V4U4).
    Predicted N-terminus: Arg 58
  • Molecular Characterization
    Online(Arg 58 - Thr 181) Q8V4U4

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.5 kDa. The protein migrates as 15 kDa and 17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Monkeypox virus (strain Zaire-96-I-16) A35R , His Tag (Cat. No. A3R-M52H3) SDS-PAGE gel

Monkeypox virus (strain Zaire-96-I-16) A35R , His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Monkeypox virus (strain Zaire-96-I-16) A35R , His TagMonkeypox virus (strain Zaire-96-I-16) A35R , His Tag (Cat. No. A3R-M52H3) ELISA bioactivity

Immobilized Monkeypox virus (strain Zaire-96-I-16) A35R , His Tag (Cat. No. A3R-M52H3) at 5 μg/mL (100 μL/well) can bind Anti-Monkeypox-A35R-Antibody with a linear range of 0.1-3 (QC tested).

  • Background
    Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. The envelope glycoprotein A35R on the EV surface has been predicted to influence intercellular diffusion of virions.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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