Streptavidin Protein-Alexa Fluor™ 488 (MALS verified)

Synonyms

Streptavidin, SA

Source

Streptavidin Protein-Alexa Fluor 488 (STN-NA118) is expressed from E. coli cells.

Molecular Characterization

This protein carries no "tag".

The protein has a calculated MW of 13.8 kDa. The protein migrates as 15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

The protein is designed as a tetramer.

Conjugate

AF488

Excitation Wavelength: 488 nm

Emission Wavelength: 517 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.

Protein Ratio

The AF488 to protein molar ratio is 1-2.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process