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Your Position: Home > Protein > IL-12B & IL-12A > IL2-H5210

Human IL-12 Protein, premium grade

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  • Synonym
    IL12,p70,Interleukin-12
  • Source
    Human IL-12 Protein, premium grade(IL2-H5210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 & Arg 23 - Ser 219 (Accession # P29460-1 & P29459-1).
    Predicted N-terminus: Ile 23
    Human IL-12 Protein, premium grade (IL2-H5210), designed for preclinical stage, has the same activity and performance with GMP Human IL-12, which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.
  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 58.9 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-12B & IL-12A SDS-PAGE

Human IL-12 Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-12B & IL-12A MALS images

The purity of Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) is more than 95% and the molecular weight of this protein is around 57-77 kDa verified by SEC-MALS.

Bioactivity-Bioactivity CELL BASE
 IL-12B & IL-12A CELL

Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) stimulates secretion of IFN-γ by NK-92 human natural killer lymphoma cells. The specific activity of ActiveMax® Human IL-12 ,Tag Free (Cat. No. IL2-H5210) is > 1ⅹ10^7 IU/mg (QC tested).

Bioactivity-ELISA
 IL-12B & IL-12A ELISA

Immobilized Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) with a linear range of 0.005-0.156 μg/mL (QC tested).

 IL-12B & IL-12A ELISA

Immobilized Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL (Routinely tested).

Bioactivity-BLI
 IL-12B & IL-12A BLI

Loaded Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) on SA Biosensor, can bind Human IL-12 Protein, premium grade (Cat. No. IL2-H5210) with an affinity constant of 1.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.
  • Clinical and Translational Updates

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  • Latest Research Phase:Approved

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