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This protein carries no "tag".
The protein has a calculated MW of 26 kDa. The protein migrates as 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IgG1 Fc (C103S, D239E, L241E), Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IgG1 Fc (C103S, D239E, L241E), Tag Free (Cat. No. IG1-H52H1) is more than 95% and the molecular weight of this protein is around 52-70 kDa verified by SEC-MALS.
Immobilized Human IgG1 Fc (C103S, D239E, L241E), Tag Free (Cat. No. IG1-H52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.3-2 ng/mL (QC tested).
Immobilized Human IgG1 Fc (C103S, D239E, L241E), Tag Free (Cat. No. IG1-H52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176), Avitag,His Tag (Cat. No. CDA-H82E9) with a linear range of 0.02-0.625 μg/mL (Routinely tested).
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