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Your Position: Home > Receptor Tyrosine Kinases > ErbB4 > Human ErbB4 HER4 Protein, With C-Fc Tag

Human ErbB4 / Her4 Protein, Fc Tag

  • Synonym
    HER4,ErbB4,MGC138404,p180erbB4
  • Source
    Human ErbB4, Fc Tag (ER4-H5251) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Pro 651 (Accession # NP_001036064.1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    Online(Gln 26 - Pro 651) NP_001036064.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 96.0 kDa. The protein migrates as 90-106 kDa and 116-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ErbB4, Fc Tag (Cat. No. ER4-H5251) SDS-PAGE gel

Human ErbB4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human ErbB4, Fc TagHuman ErbB4, Fc Tag (Cat. No. ER4-H5251) ELISA bioactivity

Immobilized Human ErbB4, Fc Tag (Cat. No. ER4-H5251) at 2 μg/mL (100 μL/well) can bind Human NRG1-beta 1 Protein with a linear range of 0.6-10 ng/mL (Routinely tested).

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