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Your Position: Home > Antibodies > His > HIS-AY63

Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63)

  • Source
    Monoclonal Anti-His Tag Antibody,Mouse IgG1 (AY63) antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with His Tag Protein.
  • Isotype
    Mouse IgG1 | Mouse Kappa
  • Specificity
    This product is a specific antibody specifically reacts with His Tag Protein.
  • Application
    ELISA,FITC
  • Purity

    >95% as determined by SDS-PAGE.

  • Endotoxin
    Less than 1.0 EU per mg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
His SDS-PAGE

Monoclonal Anti-His Tag Antibody,Mouse IgG1 (AY63) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-Elisa
 His ELISA

Immobilized Human IL-4 R alpha / CD124 Protein, His Tag (MALS verified) (Cat. No. ILR-H5221) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-His Tag Antibody,Mouse IgG1 (AY63) (Cat. No. HIS-AY63) with a linear range of 0.20-3.13 ng/mL (QC tested)

  • Background
    His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $250.00

Price(USD) : $1580.00

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