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Product Details
Synonyms
Interleukin-24, IL-24, Melanoma differentiation-associated gene 7 protein, MDA-7, Suppression of tumorigenicity 16 protein, IL24, MDA7, ST16
Source
Human IL-24 Protein, His Tag (IL4-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 52 - Leu 206 (Accession # Q13007-1).
Predicted N-terminus: Gln 52
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.0 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-24 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-SPR
Human IL-22 R alpha 1 Superstable Mutant Protein, Fc Tag (Cat. No. IL1-H5257) captured on Protein A Chip can bind Human IL-24 Protein, His Tag (Cat. No. IL4-H52H4) with an affinity constant of 3.28 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Bioactivity-BLI
Loaded Human IL-22 R alpha 1 Protein, Fc Tag (Cat. No. IL1-H5258) on Protein A Biosensor, can bind Human IL-24 Protein, His Tag (Cat. No. IL4-H52H4) with an affinity constant of 1.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin 24
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Phase 1 Clinical
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