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Your Position: Home > Protein > CD39 > CD9-H52H5

Human CD39 / ENTPD1 (Q96A, N99A, E143A, R147E) Protein, His Tag (active enzyme, MALS verified)

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  • Synonym
    CD39,ENTPD1,NTPDase 1,Entpd1,Ecto-ATPDase 1,Ecto-ATPase 1
  • Source
    Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag(CD9-H52H5) is expressed from human 293 cells (HEK293). It contains AA Thr 38 - Val 478 (Accession # P49961-1 (Q96A, N99A, E143A, R147E)).
  • Molecular Characterization
    CD39 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 52.1 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris,150 mM NaCl,pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD39 SDS-PAGE

Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
CD39 MALS images

The purity of Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag (Cat. No. CD9-H52H5) is more than 90% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD39 ELISA

Immobilized Human CD39 / ENTPD1 (Q96A, N99A, E143A, R147E), His Tag (Cat. No. CD9-H52H5) at 1 μg/mL (100 μL/well) can bind X-IgG1 with a linear range of 0.5-3 ng/mL (Routinely tested).

Bioactivity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 8,000 pmol/min/μg (QC tested).

  • Background
    CD39 is also known as Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1, in the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells.Regulatory T cells(Tregs) mediate immunosuppression through multiple, non-redundant, cell-contact dependent and independent mechanisms, a growing body of evidence suggests an important role for the CD39-CD73-adenosine pathway. CD39 ectonucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.
  • Clinical and Translational Updates

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Price(USD) : $400.00

Price(USD) : $2600.00

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