|Standard Curve Range||0.03125 pg-2 pg|
|Assay Time||30 min|
|Suitable Sample Type||For the quantitative determination of RNase A in the environment, some biological materials, common molecular biological reagents such as reaction buffers, enzymes such as reverse transcriptase and RNA polymerase, and buffers for RNA purification and storage.|
|Sample volume||10 μL & 80 μL|
|RNase Substrate||2 nmol||10 nmol|
|10X Reaction Buffer for RNase||10 mL||10 mL|
|RNase A (10μg/mL)||100 μL||500 μL|
|TE Buffer (pH 7.0)||1.5 mL||6 mL|
|Nuclease-free Water||10 mL||50 mL|
RNase Activity Assay Kit (Fluorescence) is a convenient and sensitive assay tool to test the presence of RNase in buffers, reagents, and other components.
It is for research use only.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 3 months from the date of opening.
Add 90 μL RNase Substrate Working Solution (mix RNase Substrate, 10×Reaction Buffer and Nuclease-free Water by 1:1:7 volume) to each 96-well plate, and add 10 μL RNase A standards (0-200 pg/mL×10 μL / well = 0-2 pg/well), incubate the plate in the fluorometer (BMG CLARIOstar) collecting real-time data at one minute intervals for 30 minutes at 37°C using the settings described in this section. The RNase Activity Assay can be evaluated in rigorous kinetic terms using real-time data.
This assay kit employs a standard detection of RNase A. Add 90 μL RNase Substrate Working Solution (mix RNase Substrate, 10×Reaction Buffer and Nuclease-free Water by 1:1:7 volume) to each 96-well plate, and add 10 μL of RNase A standards (0-200 pg/mL×10 μL/well = 0-2 pg/well), incubate for 30 minutes at 37°C. Then measure the fluorescence using the settings described in this section in a fluorometer (BMG CLARIOstar). Take RFU of standards as the ordinate and RNase concentration as the abscissa. Four parameters logistic are used to draw the standard curve. This following data is for reference only.
Eight replicates of each of seven samples containing different concentrations of RNase were tested in one assay, Intra-Assay Precision CV＜10%.
Eight replicates of each of seven samples containing different concentrations of RNase were tested in eight independent assays, Inter-Assay Precision CV<15%.
The probe is freeze-thawed 3 times, the kit performance meets the requirements, the sensitivity is not reduced, and the CV< 10%.
Three different concentrations of RNase was spiked into four different kinds of matrixes. The range of the recovery rate is 80%~120%.
DNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A002) and RNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A001) were used to detect nuclease residues in the same sample. No significant cross-reactivity or interference was observed.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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