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This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 52.7 kDa. The protein migrates as 43 kDa,55 kDa and 55-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse IL-10 R alpha Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Human IL-10, His Tag (Cat. No. IL0-H4248) at 5 μg/mL (100 μL/well) can bind Mouse IL-10 R alpha Protein, Fc Tag (Cat. No. ILR-M5254) with a linear range of 0.002-0.063 μg/mL (QC tested).
Loaded Mouse IL-10, His Tag (Cat. No. IL0-M4248) on HIS1K Biosensor, can bind Mouse IL-10 R alpha Protein, Fc Tag (Cat. No. ILR-M5254) with an affinity constant of 32.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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